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Translate is a tool which allows the translation of a nucleotide (DNA/RNA) sequence to a protein sequence.
ProtParam [Documentation / Reference] is a tool which allows the computation of various physical and chemical parameters for a given protein stored in UniProtKB or for a user entered protein sequence. The computed parameters include the molecular weight, theoretical pI, amino acid composition, atomic composition, extinction coefficient ...
Compute pI/Mw is a tool which allows the computation of the theoretical isoelectric point (pI) and molecular weight (Mw) of a list of UniProtKB entries or user entered sequences.
SIM is a program which finds a user-defined number of best non-intersecting alignments between two protein sequences or within a sequence . Once the alignment is computed, you can view it using LALNVIEW, a graphical viewer program for pairwise alignments [reference to LANVIEW].
Translate - Programmatic access Parameters dna_sequence Bare nucleotide sequence output_format The output format. Only 'fasta' available. Examples to submit a form with POST Curl
FindMod is a tool that can predict potential protein post-translational modifications (PTM) and find potential single amino acid substitutions in peptides.
Analysis tools include Compute pI/Mw, a tool for predicting protein isoelectric point (pI) and molecular weight (Mw); ProtParam, to calculate various physicochemical parameters; PeptideMass, a tool for theoretically cleaving proteins and calculating the masses of their peptides and any known cellular or artifactual posttranslational modifi-
This tool calculates the estimated pI and Mw of a specified Swiss-Prot/TrEMBL entry or a user-entered AA sequence. These parameters are useful if you want to know the approximate region of a 2-D gel where a protein may be found.
For references to the individual databases and tools accessible via ExPASy, please refer to the specific subsections of ExPASy: UniProtKB; PROSITE; ENZYME; SWISS-2DPAGE; individual proteomics tools.
PeptideMass [Documentation / References] cleaves a protein sequence from the UniProt Knowledgebase (Swiss-Prot and TrEMBL) or a user-entered protein sequence with a chosen enzyme, and computes the masses of the generated peptides.