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Expression vectors are the basic tools in biotechnology for the production of proteins . The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. [1] The goal of a well-designed expression vector is the efficient ...
NdeI is a specific Type II restriction enzyme that cuts open specific target sequences, unlike exonucleases. [2] This enzyme is used in gene cloning to cut open reading frames in the plasmid of certain bacteria such as E. coli and insert a foreign gene, such as the gfpuv gene that codes for bio fluorescence of the jelly fish Aequorea victoria.
pUC19 is one of a series of plasmid cloning vectors designed by Joachim Messing and co-workers. [1] The designation "pUC" is derived from the classical "p" prefix (denoting "plasmid") and the abbreviation for the University of California, where early work on the plasmid series had been conducted. [2] The pUC plasmids are all circular double ...
Positron emission tomography ( PET) [1] is a functional imaging technique that uses radioactive substances known as radiotracers to visualize and measure changes in metabolic processes, and in other physiological activities including blood flow, regional chemical composition, and absorption. Different tracers are used for various imaging ...
PET-MRI systems don't offer a direct way to obtain attenuation maps, unlike stand-alone PET or PET-CT systems. [32] [33] Stand alone PET systems' attenuation correction (AC) is based on a transmission scan (mu - map) acquired using a 68 Ge ( Germanium-68 ) rotating rod source, which directly measures photon attenuation at 511 keV.
Novagen Software Ltd (commonly referred to as Novagen) was a British software developer which released a number of computer games on a variety of platforms from the mid-1980s to the early 1990s. The company was set up by Paul Woakes and Bruce Jordan [2] and employed approximately 18 people. [3]
Beagles were a close second, in the worst behaved category. With Havanese, French Bulldogs, and Cavalier King Charles Spaniels following close behind. The top best behaved breeds were Labs ...
T7 expression system. The T7 expression system is used in the field of microbiology to clone recombinant DNA using strains of E. coli. [1] It is the most popular system for expressing recombinant proteins in E. coli. [2] By 2021, this system had been described in over 220,000 research publications. [3]
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