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Expression vectors are the basic tools in biotechnology for the production of proteins . The vector is engineered to contain regulatory sequences that act as enhancer and promoter regions and lead to efficient transcription of the gene carried on the expression vector. [1] The goal of a well-designed expression vector is the efficient ...
T7 expression system. The T7 expression system is used in the field of microbiology to clone recombinant DNA using strains of E. coli. [1] It is the most popular system for expressing recombinant proteins in E. coli. [2] By 2021, this system had been described in over 220,000 research publications. [3]
Nde I is a specific Type II restriction enzyme that cuts open specific target sequences, unlike exonucleases. [2] This enzyme is used in gene cloning to cut open reading frames in the plasmid of certain bacteria such as E. coli and insert a foreign gene, such as the gfpuv gene that codes for bio fluorescence of the jelly fish Aequorea victoria .
Positron emission tomography ( PET) [1] is a functional imaging technique that uses radioactive substances known as radiotracers to visualize and measure changes in metabolic processes, and in other physiological activities including blood flow, regional chemical composition, and absorption. Different tracers are used for various imaging ...
PET-MRI systems don't offer a direct way to obtain attenuation maps, unlike stand-alone PET or PET-CT systems. [32] [33] Stand alone PET systems' attenuation correction (AC) is based on a transmission scan (mu - map) acquired using a 68 Ge ( Germanium-68 ) rotating rod source, which directly measures photon attenuation at 511 keV.
Novagen Software Ltd (commonly referred to as Novagen) was a British software developer which released a number of computer games on a variety of platforms from the mid-1980s to the early 1990s. The company was set up by Paul Woakes and Bruce Jordan [2] and employed approximately 18 people. [3]
proteins. PETases are an esterase class of enzymes that catalyze the breakdown (via hydrolysis) of polyethylene terephthalate (PET) plastic to monomeric mono-2-hydroxyethyl terephthalate (MHET). The idealized chemical reaction is: (ethylene terephthalate) n + H 2 O → (ethylene terephthalate) n-1 + MHET,
This is a list of positron emission tomography (PET) radiotracers. These are chemical compounds in which one or more atoms have been replaced by a short-lived, positron emitting radioisotope. Cardiology
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